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Literature summary extracted from

  • Hasegawa, H.; Nakanishi, N.
    NADPH-specific dihydropteridine reductase from bovine liver (1987), Methods Enzymol., 142, 111-116.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 2,6-dichlorophenolindophenol 0.0001 mM, 50% inhibition Bos taurus
1.5.1.34 aminopterin 1 mM, 50% inhibition, NADPH-specific enzyme Bos taurus
1.5.1.34 L-tyrosine noncompetitive vs. NADPH Bos taurus
1.5.1.34 methopterin 0.7 mM, 50% inhibition of NADPH-specific enzyme Bos taurus
1.5.1.34 NADP+ competitive vs. NADPH Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0014
-
 quinonoid 7,8-dihydro-6-methylpterin cofactor NADPH, NADPH-specific enzyme Bos taurus
1.5.1.34 0.0014
-
 NADPH reduction of quinonoid 7,8-dihydro-6-methylpterin, NADPH-specific enzyme Bos taurus
1.5.1.34 0.0017
-
 NADPH reduction of quinonoid 7,8-dihydropterin, NADPH-specific enzyme Bos taurus
1.5.1.34 0.0068
-
 quinonoid 7,8-dihydropterin cofactor NADPH, NADPH-specific enzyme Bos taurus
1.5.1.34 0.013
-
 quinonoid 7,8-dihydro-6-methylpterin cofactor NADH, NADPH-specific enzyme Bos taurus
1.5.1.34 2.9
-
 NADH reduction of quinonoid 7,8-dihydro-6-methylpterin, NADPH-specific enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 35000
-
 2 * 35000, NADPH-specific dihydropteridine reductase, SDS-PAGE Bos taurus
1.5.1.34 65000
-
 equilibrium centrifugation, NADPH-specific liver enzyme Bos taurus
1.5.1.34 68000
-
 NADPH-specific enzyme, gel filtration Bos taurus
1.5.1.34 70000
-
 NADPH-specific enzyme, native PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 0.972
-
 NADPH-specific enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Bos taurus 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-dihydropterin + NADPH + H+
-
 Bos taurus 5,6,7,8-tetrahydropterin + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer 2 * 35000, NADPH-specific dihydropteridine reductase, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus
1.5.1.34 NADPH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.0032
-
 NADP+ NADPH-specific enzyme Bos taurus